Hotspot

rpl32-trnL-UAG

SSC · 113527-114380 · 854 bp

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Pi 0.00898
MarkerSeek score 51.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
854
variable_sites
14
pi
0.00898
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAGGGGTATTGTGCATCGT TTTAGGAGATGACATGCCGC 50.0 50.0 58.0 58.0 1066-1068 bp 1.0 80.4
rpl32-trnL-UAG_p2 GAAGGGGTATTGTGCATCGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1011-1013 bp 1.0 79.9
rpl32-trnL-UAG_p3 GAAGGGGTATTGTGCATCGT AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 1004-1006 bp 1.0 79.4
rpl32-trnL-UAG_p4 GAAGGGGTATTGTGCATCGT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 1010-1012 bp 1.0 79.3
rpl32-trnL-UAG_p5 GAAGGGGTATTGTGCATCGT CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 1009-1011 bp 1.0 79.3