Hotspot

ycf4-cemA

LSC · 61600-62472 · 873 bp

Back to Anna

Pi 0.00535
MarkerSeek score 56.90
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
873
variable_sites
9
pi
0.00535
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CGTTGACCCGTACTGATGAG GAGAGAGAAATCCAACAGGACA 55.0 45.5 58.1 57.5 1037-1048 bp 1.0 60.5
ycf4-cemA_p2 CGTTGACCCGTACTGATGAG TGAGAGAGAAATCCAACAGGAC 55.0 45.5 58.1 57.5 1038-1049 bp 1.0 60.5
ycf4-cemA_p3 GAACAAAAAGCCGCCGAATT GAGAGAGAAATCCAACAGGACA 45.0 45.5 58.2 57.5 996-1007 bp 1.0 59.5
ycf4-cemA_p4 GAACAAAAAGCCGCCGAATT TGAGAGAGAAATCCAACAGGAC 45.0 45.5 58.2 57.5 997-1008 bp 1.0 59.5
ycf4-cemA_p5 CGTTGACCCGTACTGATGAG TCCAACAGGACAAAAACACT 55.0 40.0 58.1 55.3 1027-1038 bp 1.0 59.3