Hotspot

petN-psbM

LSC · 29476-30482 · 1007 bp

Back to Anna

Pi 0.0048
MarkerSeek score 57.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1007
variable_sites
9
pi
0.0048
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1080-1103 bp 1.0 74.1
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1078-1101 bp 1.0 74.1
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1076-1099 bp 1.0 73.6
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1074-1097 bp 1.0 73.6
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 1077-1100 bp 1.0 73.5