Hotspot

atpF-atpH

LSC · 13460-13809 · 350 bp

Back to Anna

Pi 0.01
MarkerSeek score 37.10
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
350
variable_sites
7
pi
0.01
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 AACAACTCACACACACTCCC GTCCTTTCGTCTCGTTTCGA 50.0 50.0 58.0 58.0 1068-1088 bp 1.0 80.2
atpF-atpH_p2 AACAACTCACACACACTCCC TCCTTTCGTCTCGTTTCGAC 50.0 50.0 58.0 58.0 1067-1087 bp 1.0 80.2
atpF-atpH_p3 AACAACTCACACACACTCCC AGTCCTTTCGTCTCGTTTCG 50.0 50.0 58.0 58.0 1069-1089 bp 1.0 80.2
atpF-atpH_p4 AACAACTCACACACACTCCC CCTTTCGTCTCGTTTCGACT 50.0 50.0 58.0 58.0 1066-1086 bp 1.0 80.2
atpF-atpH_p5 CACACACACTCCCTTTCCAA TCCTTTCGTCTCGTTTCGAC 50.0 50.0 57.9 58.0 1060-1080 bp 1.0 80.1