Hotspot

rbcL

LSC · 57166-58599 · 1434 bp

Back to Anisodus

Pi 0.00349

MarkerSeek score 60.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 5
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGG	TGGGCCGAGTTTAATTGCAA	55.0	45.0	58.1	58.4	1556 bp	1.0	76.3
rbcL_p2	TCATGAGTTGTAGGGAGGGG	TTGGGCCGAGTTTAATTGCA	55.0	45.0	58.1	58.4	1557 bp	1.0	76.3
rbcL_p3	TGAGTTGTAGGGAGGGGTTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.9	58.4	1553 bp	1.0	76.2
rbcL_p4	TGAGTTGTAGGGAGGGGTTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.9	58.4	1554 bp	1.0	76.2
rbcL_p5	ATGAGTTGTAGGGAGGGGTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.6	58.4	1554 bp	1.0	74.3