Hotspot

ndhF

SSC · 112441-114663 · 2223 bp

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Pi 0.00225

MarkerSeek score 43.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 5
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGGAATCGACCATTTCGCT	TGAATCCCCGTTCGATTCAC	45.0	50.0	57.7	58.0	2806-2810 bp	1.0	77.7
ndhF_p2	ATGGAATCGACCATTTCGCT	AATCCCCGTTCGATTCACTG	45.0	50.0	57.7	58.0	2804-2808 bp	1.0	77.7
ndhF_p3	TGAACTGGACAATCAGTGGG	TGAATCCCCGTTCGATTCAC	50.0	50.0	57.4	58.0	2605-2609 bp	1.0	75.9
ndhF_p4	GAACTGGACAATCAGTGGGT	TGAATCCCCGTTCGATTCAC	50.0	50.0	57.4	58.0	2604-2608 bp	1.0	75.9
ndhF_p5	TGAACTGGACAATCAGTGGG	AATCCCCGTTCGATTCACTG	50.0	50.0	57.4	58.0	2603-2607 bp	1.0	75.9