Hotspot

atpI

LSC · 15019-15762 · 744 bp

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Pi 0.00403

MarkerSeek score 61.10

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 3
pi: 0.00403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TGAGCCACCCACCATATACA	TTTTCAGAGGGCAAGGCAAT	50.0	45.0	58.1	58.0	822 bp	1.0	79.3
atpI_p2	AATTCTCTTGAGCCACCCAC	TTTTCAGAGGGCAAGGCAAT	50.0	45.0	57.8	58.0	830 bp	1.0	78.5
atpI_p3	TGAGCCACCCACCATATACA	TTTTTCAGAGGGCAAGGCAA	50.0	45.0	58.1	58.2	823 bp	1.0	77.7
atpI_p4	TCTTGAGCCACCCACCATAT	TTTTCAGAGGGCAAGGCAAT	50.0	45.0	58.4	58.0	825 bp	1.0	76.9
atpI_p5	AATTCTCTTGAGCCACCCAC	TTTTTCAGAGGGCAAGGCAA	50.0	45.0	57.8	58.2	831 bp	1.0	76.9