Hotspot

rpl20-rps12

LSC · 71300-72113 · 814 bp

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Pi 0.00369

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 3
pi: 0.00369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	970-972 bp	1.0	75.1
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	969-971 bp	1.0	75.1
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	933-935 bp	1.0	73.6
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	932-934 bp	1.0	73.6
rpl20-rps12_p5	TTTGTTCTACGTCTCCGAGC	AAATGTCACGAAATCCCCCG	50.0	50.0	57.7	58.5	928-930 bp	1.0	73.3