Hotspot

petA-psbJ

LSC · 65357-66419 · 1063 bp

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Pi 0.00564
MarkerSeek score 48.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1063
variable_sites
6
pi
0.00564
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGGCCGAAATGAATTTCTAG ATTCCGGATTGGGTTCATCC 40.0 50.0 53.7 57.6 1109-1129 bp 1.0 42.9
petA-psbJ_p2 TGGCCGAAATGAATTTCTAG TGGTTCATATTCCGGATTGGG 40.0 47.6 53.7 57.8 1117-1137 bp 1.0 40.4
petA-psbJ_p3 TGGCCGAAATGAATTTCTAG GGTTCATATTCCGGATTGGGT 40.0 47.6 53.7 57.8 1116-1136 bp 1.0 40.4
petA-psbJ_p4 TGGCCGAAATGAATTTCTAG TATTCCGGATTGGGTTCATCC 40.0 47.6 53.7 57.5 1110-1130 bp 1.0 40.4
petA-psbJ_p5 TGGCCGAAATGAATTTCTAG GCCGATACTACTGGAAGGAT 40.0 50.0 53.7 56.2 1203-1223 bp 1.0 40.4