Hotspot

ycf4-cemA

LSC · 62718-63473 · 756 bp

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Pi 0.00529

MarkerSeek score 61.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 4
pi: 0.00529
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GAAAGAAATCCACCAGGGCA	55.0	50.0	58.0	58.1	934-935 bp	1.0	79.5
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.1	58.1	933-934 bp	1.0	79.3
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.1	58.1	932-933 bp	1.0	79.3
ycf4-cemA_p4	CATTCCCTTGACCCGTACTG	AGAAAGAAATCCACCAGGGC	55.0	50.0	58.0	57.8	935-936 bp	1.0	78.4
ycf4-cemA_p5	TTCCCTTGACCCGTACTGAT	AGAAAGAAATCCACCAGGGC	50.0	50.0	58.1	57.8	933-934 bp	1.0	78.1