Hotspot

rpl16

LSC · 84968-86671 · 1704 bp

Back to Angraecum

Pi 0.0182
MarkerSeek score 60.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1704
variable_sites
46
pi
0.0182
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ACTCCTTCCTTCTTCTTTTACT AGGTTGAATAAAAATTTGATTTACCCT 36.4 25.9 54.4 56.5 1792 bp 0.333333 13.3
rpl16_p2 ACTCCTTCCTTCTTCTTTTACTT AGGTTGAATAAAAATTTGATTTACCCT 34.8 25.9 55.2 56.5 1792 bp 0.333333 13.3
rpl16_p3 ACTCCTTCCTTCTTCTTTTACT AGGTTGAATAAAAATTTGATTTACCC 36.4 26.9 54.4 55.2 1792 bp 0.333333 13.3
rpl16_p4 ACTCCTTCCTTCTTCTTTTACT GGTTGAATAAAAATTTGATTTACCCT 36.4 26.9 54.4 55.2 1791 bp 0.333333 13.3
rpl16_p5 ACTCCTTCCTTCTTCTTTTACTT GGTTGAATAAAAATTTGATTTACCCT 34.8 26.9 55.2 55.2 1791 bp 0.333333 13.3