Hotspot

psaC-ndhE

SSC · 117915-118740 · 826 bp

Back to Angraecum

Pi 0.0308

MarkerSeek score 67.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 826
variable_sites: 34
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AATACATCCGTGGGACAAGC	CCGAAGCATGGCTAGAGTAC	50.0	55.0	58.0	57.9	902-1051 bp	1.0	85.8
psaC-ndhE_p2	GACAGGCGGATTCACATCTT	CCGAAGCATGGCTAGAGTAC	50.0	55.0	58.0	57.9	1000-1149 bp	1.0	85.6
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	CCGAAGCATGGCTAGAGTAC	50.0	55.0	57.9	57.9	885-1034 bp	1.0	85.4
psaC-ndhE_p4	TATACCAGAAAAGCCCGTGC	CCGAAGCATGGCTAGAGTAC	50.0	55.0	58.0	57.9	1309-1340 bp	0.666667	70.0
psaC-ndhE_p5	TTCTCTTTTCTCGAGCACGG	CCGAAGCATGGCTAGAGTAC	50.0	55.0	57.9	57.9	1302 bp	0.333333	51.7