Hotspot

trnL-UAG-ccsA

SSC · 114735-114861 · 127 bp

Back to Angraecum

Pi 0.042

MarkerSeek score 68.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 127
variable_sites: 8
pi: 0.042
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTCTAAGGTCGCAAATATCA	50.0	40.9	57.9	57.6	211-234 bp	1.0	62.7
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	TGCTCTAAGGTCGCAAATATCA	45.0	40.9	57.8	57.6	218-241 bp	1.0	62.1
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	TGCTCTAAGGTCGCAAATATCA	55.0	40.9	57.8	57.6	213-236 bp	1.0	62.1
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	TGCTCTAAGGTCGCAAATATCA	55.0	40.9	57.8	57.6	212-235 bp	1.0	62.1
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	TGCTCTAAGGTCGCAAATATCA	50.0	40.9	58.3	57.6	217-240 bp	1.0	61.5