Hotspot

trnN-GUU-rpl32

SSC · 113391-113930 · 540 bp

Back to Angraecum

Pi 0.0417
MarkerSeek score 75.80
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
540
variable_sites
30
pi
0.0417
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG TTTGACATAGAAGTACGTTTTT 50.0 27.3 58.1 52.0 593-618 bp 1.0 48.0
trnN-GUU-rpl32_p2 AGTCAGTTAACAGCCAACCG TTTTGACATAGAAGTACGTTTT 50.0 27.3 58.1 52.0 594-619 bp 1.0 48.0
trnN-GUU-rpl32_p3 AGTCAGTTAACAGCCAACCG TTTTTGACATAGAAGTACGTTT 50.0 27.3 58.1 52.0 595-620 bp 1.0 48.0
trnN-GUU-rpl32_p4 ACGACCAGTCAGTTAACAGC TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.8 52.0 599-624 bp 1.0 47.9
trnN-GUU-rpl32_p5 ACGACCAGTCAGTTAACAGC TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.8 52.0 600-625 bp 1.0 47.9