Hotspot

rpoA-rps11

LSC · 82562-82652 · 91 bp

Back to Angraecum

Pi 0.0321

MarkerSeek score 73.10

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 4
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACGCTTGCTGTCTACTCTTG	AAGAGATGCGGCATTACGAG	50.0	50.0	57.9	58.1	276-284 bp	1.0	81.7
rpoA-rps11_p2	ACGCTTGCTGTCTACTCTTG	GAAGAGATGCGGCATTACGA	50.0	50.0	57.9	58.1	277-285 bp	1.0	81.7
rpoA-rps11_p3	ACGCTTGCTGTCTACTCTTG	CGTACGTGATGTAACACCCA	50.0	50.0	57.9	57.6	225-233 bp	1.0	80.8
rpoA-rps11_p4	GGACAGAACGAAACGACCAT	AAGAGATGCGGCATTACGAG	50.0	50.0	58.2	58.1	303-311 bp	1.0	80.3
rpoA-rps11_p5	GGACAGAACGAAACGACCAT	GAAGAGATGCGGCATTACGA	50.0	50.0	58.2	58.1	304-312 bp	1.0	80.3