Hotspot

psbB-psbT

LSC · 76932-77570 · 639 bp

Back to Angraecum

Pi 0.0277

MarkerSeek score 63.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 22
pi: 0.0277
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	CGGGCTACTTTGAAATCCGA	GAGCCATTGCAACTCCCATA	50.0	50.0	58.0	57.9	1336-1415 bp	1.0	85.1
psbB-psbT_p2	GGGATGGAATAGCTGTTGGG	CCCTAGTCGCCATCTCCATA	55.0	55.0	58.0	58.1	1369-1458 bp	1.0	84.9
psbB-psbT_p3	GGGGATGGAATAGCTGTTGG	CCCTAGTCGCCATCTCCATA	55.0	55.0	58.0	58.1	1370-1459 bp	1.0	84.9
psbB-psbT_p4	GGGATGGAATAGCTGTTGGG	GAGCCATTGCAACTCCCATA	55.0	50.0	58.0	57.9	1638-1717 bp	1.0	84.8
psbB-psbT_p5	GGGGATGGAATAGCTGTTGG	GAGCCATTGCAACTCCCATA	55.0	50.0	58.0	57.9	1639-1718 bp	1.0	84.8