Hotspot

petA-psbJ

LSC · 64719-65871 · 1153 bp

Back to Angraecum

Pi 0.0237

MarkerSeek score 65.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 40
pi: 0.0237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	GGGTTGGGCTCATCTCTGTA	38.1	55.0	55.4	58.8	1184-1208 bp	1.0	49.9
petA-psbJ_p2	ACAGTTTGAAAAGGTTCAGTTGT	GGGTTGGGCTCATCTCTGTA	34.8	55.0	57.3	58.8	1190-1214 bp	1.0	48.8
petA-psbJ_p3	AGGTTCAGTTGTACGAAATGA	GGGTTGGGCTCATCTCTGTA	38.1	55.0	55.2	58.8	1179-1203 bp	1.0	48.1
petA-psbJ_p4	TGAAAAGGTTCAGTTGTACGAA	GGGTTGGGCTCATCTCTGTA	36.4	55.0	56.2	58.8	1184-1208 bp	1.0	48.0
petA-psbJ_p5	TTGAAAAGGTTCAGTTGTACGA	GGGTTGGGCTCATCTCTGTA	36.4	55.0	56.2	58.8	1185-1209 bp	1.0	48.0