Hotspot

psbC-trnS-UGA

LSC · 37207-37356 · 150 bp

Back to Angraecum

Pi 0.0253

MarkerSeek score 70.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 150
variable_sites: 5
pi: 0.0253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	266-272 bp	1.0	81.8
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	246-252 bp	1.0	81.3
psbC-trnS-UGA_p3	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	303-309 bp	1.0	81.0
psbC-trnS-UGA_p4	TTCCATGACCCCTCTTAGCT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.7	58.0	258-264 bp	1.0	81.0
psbC-trnS-UGA_p5	TTGTGGGACATTTGTGGCAT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.3	58.0	346-352 bp	1.0	80.1