Hotspot

psbM-trnD-GUC

LSC · 30908-32036 · 1129 bp

Back to Angraecum

Pi 0.0209

MarkerSeek score 70.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1129
variable_sites: 30
pi: 0.0209
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1085-1234 bp	1.0	84.7
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1090-1239 bp	1.0	83.3
psbM-trnD-GUC_p3	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1089-1238 bp	1.0	82.3
psbM-trnD-GUC_p4	CAGCGCAGTAGCAATAAACG	GCCATGCATATAGCTGGGAT	50.0	50.0	57.9	57.6	1108-1257 bp	1.0	81.9
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1091-1240 bp	1.0	81.5