Hotspot

rpoB-trnC-GCA

LSC · 27645-29094 · 1450 bp

Back to Angraecum

Pi 0.0146

MarkerSeek score 71.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1450
variable_sites: 29
pi: 0.0146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	CCGGGGATAAAGGATTTGCA	50.0	50.0	58.2	57.9	1362-1605 bp	1.0	83.6
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	ACCGGGGATAAAGGATTTGC	50.0	50.0	58.2	57.9	1363-1606 bp	1.0	83.6
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TCGAACCGGGGATAAAGGAT	50.0	50.0	58.2	58.2	1367-1610 bp	1.0	82.5
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	TTCGAACCGGGGATAAAGGA	50.0	50.0	58.2	58.4	1368-1611 bp	1.0	80.7
rpoB-trnC-GCA_p5	AAATTCTTCCGTCAAGCCCT	CCGGGGATAAAGGATTTGCA	45.0	50.0	57.4	57.9	1363-1606 bp	1.0	80.3