Hotspot

rps16

LSC · 5087-6203 · 1117 bp

Back to Angophora

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1117
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GAATCACCCACACCCTCTTC	AAACCAATGGAACGACCGAA	55.0	45.0	57.9	58.0	1268 bp	1.0	78.8
rps16_p2	AATCACCCACACCCTCTTCT	AAACCAATGGAACGACCGAA	50.0	45.0	58.3	58.0	1267 bp	1.0	77.7
rps16_p3	ATCACCCACACCCTCTTCTT	AAACCAATGGAACGACCGAA	50.0	45.0	58.3	58.0	1266 bp	1.0	77.7
rps16_p4	GAATCACCCACACCCTCTTC	TGGAACGACCGAACAAATGA	55.0	45.0	57.9	57.8	1261 bp	1.0	77.1
rps16_p5	CCCACACCCTCTTCTTTTGT	AAACCAATGGAACGACCGAA	50.0	45.0	57.6	58.0	1262 bp	1.0	76.8