Hotspot

rpoB

LSC · 25163-28384 · 3222 bp

Back to Angophora

Pi 0.00279

MarkerSeek score 56.90

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3222
variable_sites: 9
pi: 0.00279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TCTTCTGGGGCATACGATGA	ACTCATGTGACAAGCCAAGG	50.0	50.0	58.2	58.1	185 bp	1.0	79.2
rpoB_p2	TCTTCTGGGGCATACGATGA	TCATGTGACAAGCCAAGGAC	50.0	50.0	58.2	58.1	183 bp	1.0	79.2
rpoB_p3	TCTTCTGGGGCATACGATGA	CAGACTCATGTGACAAGCCA	50.0	50.0	58.2	57.8	188 bp	1.0	78.6
rpoB_p4	TCTTCTGGGGCATACGATGA	CTCATGTGACAAGCCAAGGA	50.0	50.0	58.2	57.8	184 bp	1.0	78.5
rpoB_p5	TCTTCTGGGGCATACGATGA	TCAGACTCATGTGACAAGCC	50.0	50.0	58.2	57.5	189 bp	1.0	76.3