Hotspot

psbA

LSC · 557-1618 · 1062 bp

Back to Angophora

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGAGTGTTGGTTATTGCTCCT	AGAATTCGTGTGCTTGGGAG	42.9	50.0	56.9	58.2	1178 bp	1.0	61.9
psbA_p2	AGAGTGTTGGTTATTGCTCCT	AGAGAATTCGTGTGCTTGGG	42.9	50.0	56.9	58.2	1180 bp	1.0	61.9
psbA_p3	AGAGTGTTGGTTATTGCTCCT	GAGAATTCGTGTGCTTGGGA	42.9	50.0	56.9	58.2	1179 bp	1.0	61.8
psbA_p4	AGAGTGTTGGTTATTGCTCCT	AATTCGTGTGCTTGGGAGTC	42.9	50.0	56.9	58.5	1176 bp	1.0	59.6
psbA_p5	AGAGTGTTGGTTATTGCTCCT	GAATTCGTGTGCTTGGGAGT	42.9	50.0	56.9	58.5	1177 bp	1.0	59.6