Hotspot

matK

LSC · 2191-3708 · 1518 bp

Back to Angophora

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATTTCCATGATCTCATAGCCA	CCTTGTTTTGACTGTATCGCA	39.1	42.9	57.7	56.8	1639 bp	1.0	40.0
matK_p2	ACATTTCCATGATCTCATAGCCA	ACCTTGTTTTGACTGTATCGC	39.1	42.9	57.7	56.8	1640 bp	1.0	40.0
matK_p3	ACATTTCCATGATCTCATAGCCA	CCTTGTTTTGACTGTATCGCAC	39.1	45.5	57.7	58.2	1639 bp	1.0	40.0
matK_p4	ACATTTCCATGATCTCATAGCCA	ACCTTGTTTTGACTGTATCGCA	39.1	40.9	57.7	58.5	1640 bp	1.0	40.0
matK_p5	ACATTTCCATGATCTCATAGCCA	TGTTTTGACTGTATCGCACT	39.1	40.0	57.7	55.3	1636 bp	1.0	40.0