Hotspot

atpA

LSC · 11246-12769 · 1524 bp

Back to Angophora

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ACGCAAAGATGAAGAGTGATCA	TGTTTGGGGCGATGAAAGAA	40.9	45.0	58.1	58.0	1650 bp	1.0	63.3
atpA_p2	ACGCAAAGATGAAGAGTGATCA	ATGTTTGGGGCGATGAAAGA	40.9	45.0	58.1	57.8	1651 bp	1.0	61.7
atpA_p3	ACGCAAAGATGAAGAGTGATCA	CATGTTTGGGGCGATGAAAG	40.9	50.0	58.1	57.7	1652 bp	1.0	61.2
atpA_p4	CGCAAAGATGAAGAGTGATCA	TGTTTGGGGCGATGAAAGAA	42.9	45.0	56.4	58.0	1649 bp	1.0	58.8
atpA_p5	ACGCAAAGATGAAGAGTGATC	TGTTTGGGGCGATGAAAGAA	42.9	45.0	56.4	58.0	1650 bp	1.0	58.8