Hotspot

rps15-ycf1

SSC · 128822-129371 · 550 bp

Back to Angophora

Pi 0

MarkerSeek score 62.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 550
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTGAAATTCAACGGACCCCC	AATAATGGGAGCCGCTTCAC	50.0	50.0	58.4	58.3	654-688 bp	1.0	78.4
rps15-ycf1_p2	TTGAAATTCAACGGACCCCC	CAATAATGGGAGCCGCTTCA	50.0	50.0	58.4	58.3	655-689 bp	1.0	78.4
rps15-ycf1_p3	TTGAAATTCAACGGACCCCC	TAATGGGAGCCGCTTCACTA	50.0	50.0	58.4	58.5	652-686 bp	1.0	76.9
rps15-ycf1_p4	GGACCCCCTGTTTTCTTCTT	AATAATGGGAGCCGCTTCAC	50.0	50.0	57.3	58.3	642-676 bp	1.0	76.0
rps15-ycf1_p5	GGACCCCCTGTTTTCTTCTT	CAATAATGGGAGCCGCTTCA	50.0	50.0	57.3	58.3	643-677 bp	1.0	76.0