Hotspot

atpF-atpH

LSC · 14154-14704 · 551 bp

Back to Angophora

Pi 0

MarkerSeek score 57.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	GGTTGTAGCCTTAGCGCTTT	50.0	50.0	58.0	58.5	634-635 bp	1.0	75.4
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	ATTGGTTGTAGCCTTAGCGC	50.0	50.0	58.0	58.6	637-638 bp	1.0	74.9
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	704-705 bp	1.0	74.3
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	705-706 bp	1.0	74.2
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	706-707 bp	1.0	74.2