Hotspot

ACOS_CP_t004-ACOS_CP_t005

LSC · 8932-9722 · 791 bp

Back to Angophora

Pi 0

MarkerSeek score 40.00

Primer available yes

Label ACOS_CP_t004-ACOS_CP_t005

Feature Metadata

label_name: ACOS_CP_t004-ACOS_CP_t005
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ACOS_CP_t004-ACOS_CP_t005_p1	CAATCCGACGCTTTAGTCCA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	904-907 bp	1.0	76.5
ACOS_CP_t004-ACOS_CP_t005_p2	AATCCGACGCTTTAGTCCAC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	903-906 bp	1.0	76.5
ACOS_CP_t004-ACOS_CP_t005_p3	ACGGAAAGAGAGGGATTCGA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.2	57.6	958-961 bp	1.0	75.3
ACOS_CP_t004-ACOS_CP_t005_p4	AGCAATCCGACGCTTTAGTC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.4	57.6	906-909 bp	1.0	73.6
ACOS_CP_t004-ACOS_CP_t005_p5	CGACGCTTTAGTCCACTCAG	AAATCAAACCGAGGGATCCC	55.0	50.0	58.4	57.6	899-902 bp	1.0	73.5