Hotspot

ACOS_CP_t001-psbA

LSC · 84-556 · 473 bp

Back to Angophora

Pi 0
MarkerSeek score 37.40
Primer available yes
Label ACOS_CP_t001-psbA

Feature Metadata

label_name
ACOS_CP_t001-psbA
feature_type
igs
region
LSC
length_bp
473
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ACOS_CP_t001-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 540 bp 1.0 78.4
ACOS_CP_t001-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 554 bp 1.0 78.4
ACOS_CP_t001-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 553 bp 1.0 78.4
ACOS_CP_t001-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 555 bp 1.0 78.4
ACOS_CP_t001-psbA_p5 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 543 bp 1.0 78.4