Hotspot

ycf66

LSC · 36066-37119 · 1054 bp

Back to Angiopteris

Pi 0.00253

MarkerSeek score 60.40

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: LSC
length_bp: 1054
variable_sites: 4
pi: 0.00253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	TCTCATCCAAGATTAACTCCAAAGA	AGAAAAGTCTATTCGAGATAGCCT	36.0	37.5	57.7	57.0	1185-1186 bp	1.0	40.3
ycf66_p2	TCTCATCCAAGATTAACTCCA	AGAAAAGTCTATTCGAGATAGCCT	38.1	37.5	53.7	57.0	1185-1186 bp	1.0	40.3
ycf66_p3	TCTCATCCAAGATTAACTCCAA	AGAAAAGTCTATTCGAGATAGCCT	36.4	37.5	54.5	57.0	1185-1186 bp	1.0	40.3
ycf66_p4	TTCTCATCCAAGATTAACTCCA	AGAAAAGTCTATTCGAGATAGCCT	36.4	37.5	54.5	57.0	1186-1187 bp	1.0	40.3
ycf66_p5	CTCATCCAAGATTAACTCCAAAGA	AGAAAAGTCTATTCGAGATAGCCT	37.5	37.5	56.4	57.0	1184-1185 bp	1.0	40.3