Hotspot

rbcL

LSC · 60605-62032 · 1428 bp

Back to Angiopteris

Pi 0.00187

MarkerSeek score 59.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 4
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GACCTCACCCTTGCAAGAAT	TGGAAGAAAAATTTGAATAAGACACA	50.0	26.9	57.8	56.3	1505 bp	1.0	40.2
rbcL_p2	GACCTCACCCTTGCAAGAAT	TGGAAGAAAAATTTGAATAAGACACAG	50.0	29.6	57.8	57.3	1505 bp	1.0	40.2
rbcL_p3	TTGAGTTGTAGGGAGGGACC	TGGAAGAAAAATTTGAATAAGACACA	55.0	26.9	58.3	56.3	1479 bp	1.0	40.2
rbcL_p4	TTGAGTTGTAGGGAGGGACC	TGGAAGAAAAATTTGAATAAGACACAG	55.0	29.6	58.3	57.3	1479 bp	1.0	40.2
rbcL_p5	GACCTCACCCTTGCAAGAAT	TCCAAAAGAATAGTAAGAAAGAGAGT	50.0	30.8	57.8	56.1	1586 bp	1.0	40.2