Hotspot

petD

LSC · 80246-81369 · 1124 bp

Back to Angiopteris

Pi 0.00237

MarkerSeek score 36.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1124
variable_sites: 4
pi: 0.00237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGATCGAATTTCCAATCTCGA	TGGTATTAGAGAAAACGCATTGG	40.9	39.1	57.6	57.2	1222-1223 bp	1.0	40.3
petD_p2	TGGATCGAATTTCCAATCTCGA	GGTATTAGAGAAAACGCATTGGT	40.9	39.1	57.6	57.2	1221-1222 bp	1.0	40.3
petD_p3	TGGATCGAATTTCCAATCTCGA	AGGACTAGATCTGATACCGT	40.9	45.0	57.6	54.2	1244-1245 bp	1.0	40.3
petD_p4	TGGATCGAATTTCCAATCTCGA	CCGTATTGGTATTAGAGAAAACGC	40.9	41.7	57.6	58.0	1228-1229 bp	1.0	40.3
petD_p5	TGGATCGAATTTCCAATCTCGA	TTGGTATTAGAGAAAACGCATTGG	40.9	37.5	57.6	57.8	1223-1224 bp	1.0	40.3