Hotspot

ndhE

SSC · 118245-118547 · 303 bp

Back to Angiopteris

Pi 0.0066

MarkerSeek score 56.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 3
pi: 0.0066
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCTCTATCTTCATCCGTTCGA	AGCTTCGGAATCTAAAGAGGA	42.9	42.9	55.7	56.2	515 bp	1.0	40.5
ndhE_p2	TCTCTATCTTCATCCGTTCGAA	AGCTTCGGAATCTAAAGAGGA	40.9	42.9	56.5	56.2	515 bp	1.0	40.5
ndhE_p3	TCTCTATCTTCATCCGTTCGA	AAGCTTCGGAATCTAAAGAGGA	42.9	40.9	55.7	56.9	516 bp	1.0	40.5
ndhE_p4	ATCTCTATCTTCATCCGTTCGA	AGCTTCGGAATCTAAAGAGGA	40.9	42.9	56.3	56.2	516 bp	1.0	40.5
ndhE_p5	TCTCTATCTTCATCCGTTCGA	AGCTTCGGAATCTAAAGAGGAT	42.9	40.9	55.7	56.7	515 bp	1.0	40.5