Hotspot

atpA

LSC · 18914-20440 · 1527 bp

Back to Angiopteris

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGGTTCAAATCCTATTGGACGT	TGCACTCACGCATGATTGAT	40.9	45.0	57.4	58.3	1750 bp	1.0	57.5
atpA_p2	AGGTTCAAATCCTATTGGACGT	TGAATTGCACTCACGCATGA	40.9	45.0	57.4	58.5	1755 bp	1.0	55.8
atpA_p3	AGGTTCAAATCCTATTGGACGT	TTGCACTCACGCATGATTGA	40.9	45.0	57.4	58.5	1751 bp	1.0	55.8
atpA_p4	AGGTTCAAATCCTATTGGACGT	ATGAATTGCACTCACGCATG	40.9	45.0	57.4	57.4	1756 bp	1.0	55.1
atpA_p5	AGGTTCAAATCCTATTGGACGT	ATTGCACTCACGCATGATTG	40.9	45.0	57.4	57.4	1752 bp	1.0	55.1