Hotspot

trnL-CAA-trnI-CAU

IRa · 151148-153665 · 2518 bp

Back to Angiopteris

Pi 0.00371
MarkerSeek score 59.00
Primer available yes
Label trnL-CAA-trnI-CAU

Feature Metadata

label_name
trnL-CAA-trnI-CAU
feature_type
igs
region
IRa
length_bp
2518
variable_sites
14
pi
0.00371
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-trnI-CAU_p1 GGTGAAATGGTAGACACGCA TGAACCTGTGAATTCGCCAA 50.0 45.0 58.2 58.0 2649-2922 bp 1.0 78.6
trnL-CAA-trnI-CAU_p2 GGTGAAATGGTAGACACGCA TTGAACCTGTGAATTCGCCA 50.0 45.0 58.2 58.0 2650-2923 bp 1.0 78.6
trnL-CAA-trnI-CAU_p3 TGGTGAAATGGTAGACACGC TGAACCTGTGAATTCGCCAA 50.0 45.0 58.2 58.0 2650-2923 bp 1.0 78.6
trnL-CAA-trnI-CAU_p4 TGGTGAAATGGTAGACACGC TTGAACCTGTGAATTCGCCA 50.0 45.0 58.2 58.0 2651-2924 bp 1.0 78.6
trnL-CAA-trnI-CAU_p5 GGTGAAATGGTAGACACGCA TAACCGTTCAGCCATGGATG 50.0 50.0 58.2 58.0 2612-2885 bp 1.0 78.5