Hotspot

rpl32-trnP-GGG

SSC · 113983-114391 · 409 bp

Back to Angiopteris

Pi 0.00489
MarkerSeek score 55.80
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
SSC
length_bp
409
variable_sites
3
pi
0.00489
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 GCCTCAAAAGCTTTTTCTTTAGC GAGGTCGTGGGTTCAAATCC 39.1 55.0 57.4 58.5 539-540 bp 1.0 47.4
rpl32-trnP-GGG_p2 GCCTCAAAAGCTTTTTCTTTAGC GGAGGTCGTGGGTTCAAATC 39.1 55.0 57.4 58.5 540-541 bp 1.0 47.4
rpl32-trnP-GGG_p3 GCCTCAAAAGCTTTTTCTTTAGC ATCTTGGGGTGATGGAGGTC 39.1 55.0 57.4 58.8 553-554 bp 1.0 45.5
rpl32-trnP-GGG_p4 TGCCTCAAAAGCTTTTTCTT GAGGTCGTGGGTTCAAATCC 35.0 55.0 54.2 58.5 540-541 bp 1.0 45.4
rpl32-trnP-GGG_p5 TGCCTCAAAAGCTTTTTCTT GGAGGTCGTGGGTTCAAATC 35.0 55.0 54.2 58.5 541-542 bp 1.0 45.4