Hotspot

rps14-psaB

LSC · 44726-44934 · 209 bp

Back to Angiopteris

Pi 0.00641

MarkerSeek score 59.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 2
pi: 0.00641
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TCCTCTTCTTCTCCCGTTCA	CGCTTCCACATCAGGGAAAT	50.0	50.0	57.7	58.2	272-277 bp	1.0	77.6
rps14-psaB_p2	TCCTCTTCTTCTCCCGTTCA	CCACATCAGGGAAATTCGGA	50.0	50.0	57.7	57.6	267-272 bp	1.0	76.3
rps14-psaB_p3	TCCTCTTCTTCTCCCGTTCA	TCCACATCAGGGAAATTCGG	50.0	50.0	57.7	57.6	268-273 bp	1.0	76.3
rps14-psaB_p4	TCCTCTTCTTCTCCCGTTCA	TAATCGCTTCCACATCAGGG	50.0	50.0	57.7	57.4	276-281 bp	1.0	74.6
rps14-psaB_p5	TCCTCTTCTTCTCCCGTTCA	CCACATCAGGGAAATTCGGAT	50.0	47.6	57.7	58.1	267-272 bp	1.0	71.1