Hotspot

psbD-trnT-GGU

LSC · 42306-43649 · 1344 bp

Back to Angiopteris

Pi 0.00199

MarkerSeek score 60.20

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1344
variable_sites: 4
pi: 0.00199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GAAGACGAAACGGTCTCTCC	GGCGTAAGTCATCGGTTCAA	55.0	50.0	58.0	58.3	1468-1469 bp	1.0	77.9
psbD-trnT-GGU_p2	AACCTACGAAGACGAAACGG	GGCGTAAGTCATCGGTTCAA	50.0	50.0	57.9	58.3	1475-1476 bp	1.0	77.6
psbD-trnT-GGU_p3	CGAAACGGTCTCTCCTTAGC	GGCGTAAGTCATCGGTTCAA	55.0	50.0	58.1	58.3	1463-1464 bp	1.0	77.4
psbD-trnT-GGU_p4	AAGCAATAGACCGGACCAAC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	57.9	58.3	1492-1493 bp	1.0	77.2
psbD-trnT-GGU_p5	GGTCTCTCCTTAGCCAGTCA	GGCGTAAGTCATCGGTTCAA	55.0	50.0	58.1	58.3	1457-1458 bp	1.0	76.9