Hotspot

ycf2

LSC · 87604-93906 · 6303 bp

Back to Angelica

Pi 0.00702

MarkerSeek score 48.30

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6303
variable_sites: 307
pi: 0.00702
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGGGCTCCTAGAATATGGCA	TGGTTCCACTCTGCAAGAAC	50.0	50.0	57.9	58.0	160-178 bp	1.0	90.8
ycf2_p2	TGGGCTCCTAGAATATGGCA	CACTCTGCAAGAACTCCGAA	50.0	50.0	57.9	57.8	154-172 bp	1.0	89.8
ycf2_p3	TGGGCTCCTAGAATATGGCA	GTGTCTGGTACTGCATGGTT	50.0	50.0	57.9	57.8	175-193 bp	1.0	89.6
ycf2_p4	TGGGCTCCTAGAATATGGCA	CATGGTTCCACTCTGCAAGA	50.0	50.0	57.9	57.8	162-180 bp	1.0	89.5
ycf2_p5	TGGGCTCCTAGAATATGGCA	CTGCAGGGTCCCAAATGAAT	50.0	50.0	57.9	58.1	238-256 bp	1.0	88.7