Hotspot

ycf1

SSC · 125447-130951 · 5505 bp

Back to Angelica

Pi 0.00852

MarkerSeek score 52.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5505
variable_sites: 289
pi: 0.00852
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCGCGATTTTTATCCACGT	ACATGCCGAAGTGATGGAAA	45.0	45.0	58.0	57.8	1079-1181 bp	1.0	87.1
ycf1_p2	TCCGCGATTTTTATCCACGT	GATGGAATCGCCCATTACGA	45.0	50.0	58.0	57.8	1162-1264 bp	1.0	87.0
ycf1_p3	CACTCATCGAAGTCCTCAGC	GATGGAATCGCCCATTACGA	55.0	50.0	58.1	57.8	1327-1429 bp	1.0	86.4
ycf1_p4	TACGCCTTTGAACACGCATA	GATGGAATCGCCCATTACGA	45.0	50.0	57.9	57.8	966-1032 bp	1.0	86.3
ycf1_p5	TGCGCATTTGCTTGAAACAA	GATGGAATCGCCCATTACGA	40.0	50.0	58.1	57.8	1003-1069 bp	0.931034	83.8