Hotspot

ndhF

SSC · 111483-113729 · 2247 bp

Back to Angelica

Pi 0.00578

MarkerSeek score 47.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 90
pi: 0.00578
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCATGAAGGCCTACCGTTG	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.3	58.3	2359-2450 bp	0.551724	61.6
ndhF_p2	AATTGGAATTCCTGTCGGGC	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2383-2474 bp	0.551724	60.1
ndhF_p3	ATGAAGGCCTACCGTTGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.8	58.3	2356-2447 bp	0.551724	58.0
ndhF_p4	CTCATGAAGGCCTACCGTTG	GGGTAGGATCCGGTAAATCG	55.0	55.0	58.3	57.3	2357-2448 bp	0.551724	57.8
ndhF_p5	CTCATGAAGGCCTACCGTTG	TTGAAAGGGGGTAGGATCCG	55.0	55.0	58.3	58.8	2365-2456 bp	0.551724	57.3