Hotspot

accD

LSC · 59187-60656 · 1470 bp

Back to Angelica

Pi 0.00549

MarkerSeek score 66.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1470
variable_sites: 83
pi: 0.00549
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CATGCAAAGCAAATAGGGGG	ATGTCAACAAAGAAACCCCA	50.0	40.0	57.4	55.3	1566-1596 bp	0.965517	55.7
accD_p2	CATGCAAAGCAAATAGGGGG	TGTCAACAAAGAAACCCCAT	50.0	40.0	57.4	55.3	1565-1595 bp	0.965517	55.7
accD_p3	CATGCAAAGCAAATAGGGGG	GTCAACAAAGAAACCCCATTCA	50.0	40.9	57.4	57.3	1564-1594 bp	0.965517	55.6
accD_p4	CATGCAAAGCAAATAGGGGG	TGTCAACAAAGAAACCCCATTC	50.0	40.9	57.4	57.3	1565-1595 bp	0.965517	55.6
accD_p5	AGCAAATAGGGGGCAAGAAA	TGTCAACAAAGAAACCCCAT	45.0	40.0	57.4	55.3	1558-1588 bp	0.965517	55.6