Hotspot

ndhF-rpl32

SSC · 113730-114772 · 1043 bp

Back to Angelica

Pi 0.0119
MarkerSeek score 63.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1043
variable_sites
73
pi
0.0119
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGCGGGTCGATCAAGTAAC TGACAAACAGGTTGATGGGG 50.0 50.0 58.0 58.0 1702-1781 bp 1.0 88.3
ndhF-rpl32_p2 AAGCGGGTCGATCAAGTAAC AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 1951-2044 bp 0.965517 87.2
ndhF-rpl32_p3 AAGCGGGTCGATCAAGTAAC ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 1950-2043 bp 0.965517 87.2
ndhF-rpl32_p4 CCGTGTGAACCAAAACCCTA AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 2179-2272 bp 0.965517 86.7
ndhF-rpl32_p5 CCGTGTGAACCAAAACCCTA ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 2178-2271 bp 0.965517 86.7