Hotspot

ndhC-trnV-UAC

LSC · 52291-53369 · 1079 bp

Back to Angelica

Pi 0.00921

MarkerSeek score 68.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1079
variable_sites: 63
pi: 0.00921
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1118-1283 bp	0.965517	84.9
ndhC-trnV-UAC_p2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1105-1270 bp	1.0	82.5
ndhC-trnV-UAC_p3	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.3	58.1	1113-1278 bp	1.0	81.5
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.8	58.1	1100-1265 bp	1.0	80.7
ndhC-trnV-UAC_p5	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1103-1268 bp	0.965517	80.3