Hotspot

trnE-UUC-trnT-GGU

LSC · 32169-32931 · 763 bp

Back to Angelica

Pi 0.0208

MarkerSeek score 72.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 73
pi: 0.0208
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	60.0	50.0	58.2	58.0	327-1042 bp	1.0	89.1
trnE-UUC-trnT-GGU_p2	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.2	57.9	292-1007 bp	1.0	88.6
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	292-1007 bp	0.965517	88.2
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	257-972 bp	0.965517	87.7
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	289-1004 bp	0.965517	87.0