Hotspot

atpH-atpI

LSC · 14458-15377 · 920 bp

Back to Angelica

Pi 0.00852

MarkerSeek score 64.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 49
pi: 0.00852
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	799-1246 bp	1.0	86.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	855-1302 bp	1.0	85.8
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	815-1262 bp	1.0	85.6
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	800-1247 bp	1.0	85.3
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.2	57.9	859-1306 bp	1.0	85.1