Hotspot

trnS-GCU-trnG-UCC

LSC · 9418-9983 · 566 bp

Back to Angelica

Pi 0.0136

MarkerSeek score 63.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 44
pi: 0.0136
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	45.0	55.0	58.0	58.1	622-671 bp	1.0	88.7
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	GGGGGTTACTCGAACGAATC	50.0	55.0	58.2	58.1	671-720 bp	1.0	87.4
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTCGAACGAATC	50.0	55.0	57.6	58.1	608-657 bp	1.0	85.9
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	45.0	50.0	58.0	58.4	623-672 bp	1.0	85.8
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GGGGGTTACTCGAACGAATC	55.0	55.0	58.5	58.1	603-652 bp	1.0	85.1