Hotspot

ycf2

Genome · 31110-36722 · 5613 bp

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Pi 0.0589
MarkerSeek score 68.30
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
5613
variable_sites
330
pi
0.0589
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 CCAATTCGTTGGTCTTTCGC ATCTGCTCCGATGGTAGTGA 50.0 50.0 58.0 57.9 760 bp 0.5 59.1
ycf2_p2 AAGGATCTCATCGAGTCGGT ATCTGCTCCGATGGTAGTGA 50.0 50.0 57.9 57.9 1085 bp 0.5 58.9
ycf2_p3 CCAATTCGTTGGTCTTTCGC ACACGGGATTGATTGGAAGG 50.0 50.0 58.0 57.9 501 bp 0.5 58.7
ycf2_p4 TTCGAAAACAAACTCGCAGC ATCTGCTCCGATGGTAGTGA 45.0 50.0 57.9 57.9 901 bp 0.5 58.6
ycf2_p5 AAGGATCTCATCGAGTCGGT ACACGGGATTGATTGGAAGG 50.0 50.0 57.9 57.9 826 bp 0.5 58.5