Hotspot

ycf1

Genome · 106729-109983 · 3255 bp

Pi 0.0687

MarkerSeek score 72.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 3255
variable_sites: 223
pi: 0.0687
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAGTCGCTACTATCGCTAC	CTGGCAAGGAAAGCGATTTG	55.0	50.0	58.0	58.0	322 bp	0.5	59.5
ycf1_p2	GCAGTCGCTACTATCGCTAC	CTGCTACTTAACTGCCTGGG	55.0	55.0	58.0	58.0	776 bp	0.5	59.4
ycf1_p3	GCAGTCGCTACTATCGCTAC	CCATCTGAAACCGTGGCATA	55.0	50.0	58.0	58.0	866 bp	0.5	59.3
ycf1_p4	ACCGAAAAGGATGTTTCCCC	CTGGCAAGGAAAGCGATTTG	50.0	50.0	58.1	58.0	1016 bp	0.5	59.2
ycf1_p5	CGAAAAGGATGTTTCCCCCA	CTGGCAAGGAAAGCGATTTG	50.0	50.0	58.1	58.0	1014 bp	0.5	59.2