Hotspot

rpl20-rps12

Genome · 68492-69286 · 795 bp

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Pi 0.0502
MarkerSeek score 81.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
Genome
length_bp
795
variable_sites
37
pi
0.0502
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TGCCACATAACCACGTTTCA TGGAACAAAATCTCCTGCCC 45.0 50.0 58.0 58.1 832-889 bp 1.0 86.6
rpl20-rps12_p2 TGCCACATAACCACGTTTCA CTCAACGTCGAGGAGTTTGT 45.0 50.0 58.0 57.9 800-857 bp 1.0 86.2
rpl20-rps12_p3 TGCCACATAACCACGTTTCA GGAACAAAATCTCCTGCCCT 45.0 50.0 58.0 57.8 831-888 bp 1.0 85.4
rpl20-rps12_p4 TGCCACATAACCACGTTTCA CTCCTGCCCTTAAAGGATGC 45.0 55.0 58.0 58.3 821-878 bp 1.0 84.7
rpl20-rps12_p5 GCCACATAACCACGTTTCAC TGGAACAAAATCTCCTGCCC 50.0 50.0 57.7 58.1 831-888 bp 1.0 84.2